Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. In mammalian cells, the EZ family group of proteins among others are responsible for the addition of one, two or three methyl groups at lysine 17 of histone H3.
Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 11ml||1 x 22ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adomet||1 x 25µl||1 x 50µl|
|Biotinylated Substrate, 25 µg/mL||1 x 100µl||1 x 200µl|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Control Enzyme (150 µg/ml)||1 x 10µl||1 x 20µl|
|Detection Antibody, 100 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 6ml||1 x 12ml|
|Histone Assay Buffer||1 x 1.5ml||1 x 3ml|
|HMT Standard, 10 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3K27 specific histone methyltrasferase activity was measured.
ab113454 has not yet been referenced specifically in any publications.