Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454)

Overview

  • Product nameHistone H3 (K27) Methyltransferase Activity Quantification Assay Kit
    See all Histone Methyltransferase kits
  • Assay typeQuantitative
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. In mammalian cells, the EZ family group of proteins among others are responsible for the addition of one, two or three methyl groups at lysine 17 of histone H3.

    Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

     

  • Tested applicationsFunctional Studiesmore details

Properties

  • Alternative names
    • HMT

Applications

Our Abpromise guarantee covers the use of ab113454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit images

  • Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3K27 specific histone methyltrasferase activity was measured.

Protocols

References for Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454)

ab113454 has not yet been referenced specifically in any publications.

Product Wall

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I am sorry but the compositon of the components in our Episeeker Histone H3 (K27) Methytransferase Activity kit and ab113474 Episeker Nuclear Extraction kits are considered proprietary and I am unable to share ...

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The strip wells are coated with streptavidin but unfortunately they are not available separately.

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We will continue to guarantee all of our products, even if they were at room temperature for several days due to shipping issues. For this kit in particular, I would expect it to still work normally even though ...

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I am sorry to hear the kit is not quite working as expected.

Could you please send me the following information in order for us to evaluate this problem:

1) The data you obtained for the stand...

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Thank you for contacting us. I have sent you a vendor information form which should contain all of the information you need. I hope this helps, please let me know if you need any other information.

I received an update from the developer of the kit shortly after I sent my previous e-mail to you. The information they provided basically confirms what I had suggested in my e-mail, except that sonication will apparently not diminish activity: 1. Yes,...

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Thank you for your inquiry. Compared to similar kits that use conventional nuclear extraction methods,  the buffer included in our nuclear extraction kit (ab 113474) contains much lower amounts of salts (20% of conventional kits) and no SDS, whi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"