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Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. H3(K9) demethylation is regulated by at least two classes of demethylases, JMJD1 (which can remove mono- and di-methylation from H3K9) and JMJD2 (which can remove trimethylation from H3K9).
Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458) allows the user to measure activity/inhibition of H3K9 specific histone demethylases using cell/tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K9 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Fluoro-Developer||1 x 4ml||1 x 8ml|
|Fluoro-Enhancer||1 x 4ml||1 x 8ml|
|HDM Assay Buffer||1 x 2ml||1 x 4ml|
|HDM Cofactor 1||1 x 25µl||1 x 50µl|
|HDM Cofactor 2||1 x 25µl||1 x 50µl|
|HDM Cofactor 3||1 x 25µl||1 x 50µl|
|HDM Standard, 10 mM||1 x 25µl||1 x 50µl|
|HDM Substrate||1 x 50µl||1 x 100µl|
Our Abpromise guarantee covers the use of ab113458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|