Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458)

Overview

  • Product nameHistone H3 (K9) Demethylase Activity Quantification Assay Kit
    See all Histone Demethylase kits
  • Assay typeEnzyme activity
  • Assay time
    1h 30m
  • Species reactivity
    Reacts with: Mouse, Human, Plants
    Predicted to work with: all Mammals, Other
  • Product overview

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. H3(K9) demethylation is regulated by at least two classes of demethylases, JMJD1 (which can remove mono- and di-methylation from H3K9) and JMJD2 (which can remove trimethylation from H3K9).

    Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458) allows the user to measure activity/inhibition of H3K9 specific histone demethylases using cell/tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K9 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113458 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458)

    This product has been referenced in:
    • Galoian K  et al. Restoration of desmosomal junction protein expression and inhibition of H3K9-specific histone demethylase activity by cytostatic proline-rich polypeptide-1 leads to suppression of tumorigenic potential in human chondrosarcoma cells. Mol Clin Oncol 3:171-178 (2015). Functional Studies ; Human . Read more (PubMed: 25469290) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"