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Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.
Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
ab113453 recognizes methylated H3 at lysine site 9.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 11ml||1 x 22ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adomet||1 x 25µl||1 x 50µl|
|Biotinylated Substrate, 25 µg/mL||1 x 100µl||1 x 200µl|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Control Enzyme (150 µg/mL)||1 x 13µl||1 x 26µl|
|Detection Antibody, 200 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 6ml||1 x 12ml|
|Histone Assay Buffer||1 x 1.5ml||1 x 3ml|
|HMT Standard, 10 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113453 has not yet been referenced specifically in any publications.