Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)


  • Product name
    Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit
    See all Histone Methyltransferase kits
  • Assay type
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.

    Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

    ab113453 recognizes methylated H3 at lysine site 9.

  • Tested applications
    Suitable for: Functional Studiesmore details



Our Abpromise guarantee covers the use of ab113453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.


  • Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.



ab113453 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.
Here are the answers to your questions:
1. Peptide.
2. Yes, avidin-coated.
3. This information cannot be disclosed currently.
4. No. unless a purified enzyme or extract with the over-expressed speci...

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A standard curve was plotted by plotting the blank corrected OD450 for three different HMT standard concentrations. This kit works well and is specific for H3K9 Me-transferase.

Abcam user community

Verified customer

Submitted Mar 10 2017

Thank you for contacting us.
Here are the answers to your questions:
1. After dilution, each diluted component should be used within the same day.
2. At 4C for same day.
3. for longer time storage, as it will not be frozen at -20C. ...

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Thank you for contacting us.
Here are the answers to your questions:
1. 100,000-500,000 cells or 10-50 mg tissues.
2. It is possible but results are not guaranteed.
3. It can be stable for 30 min after adding stop solution.
4. ...

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Thank you for contacting us and thank you for your patience.

I have heard back from the lab with the following information:

1. Yes, this kit is suitable for the conditions mentioned (rat oligodendrocytes with and without inhibitors...

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Thanks for your inquiry. Yes, the kit ab113453 is suitable for measuring H3K9 activity from various species including Drosophila based on the working principle and antibody's specificity. Please let me know if you require further ...

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