Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting.
Histone H3 (methyl K27) Assay Kit (ab115050) allows the user to specifically measure global histone H3K27 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Lysis Buffer||1 x 5ml||1 x 10ml|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Detection Antibody, 400 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 5ml||1 x 10ml|
|Extraction Buffer||1 x 8ml||1 x 16ml|
|Histone Buffer||1 x 0.5ml||1 x 1ml|
|Methylated H3K27 Control, 60 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115050 has not yet been referenced specifically in any publications.