Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.
Histone H3 (methyl K4) Assay Kit (ab115048) allows the user to specifically measure global histone H3K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Lysis Buffer||1 x 5ml||1 x 10ml|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Detection Antibody, 400 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 5ml||1 x 10ml|
|Extraction Buffer||1 x 8ml||1 x 16ml|
|Histone Buffer||1 x 0.5ml||1 x 1ml|
|Methylated H3K4 Control, 60 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115048 has not yet been referenced specifically in any publications.