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Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.
Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046) allows the user to specifically measure histone H3K4 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
|Components||96 tests||2 x 96 tests|
|10X Wash Buffer||1 x 30ml||2 x 30ml|
|30% H2O2 Solution||1 x 0.5ml||1 x 1ml|
|8-Well Control Strips||2 units||4 units|
|Antibody Buffer||1 x 10ml||1 x 20ml|
|Blocking Buffer||1 x 20ml||2 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 60µl||1 x 120µl|
|Detection Antibody, 200 µg/mL||1 x 20µl||1 x 40µl|
|Developing Solution||1 x 12ml||1 x 24ml|
|Methylated H3K4 Control, 20 µg/mL||1 x 15µl||1 x 30µl|
|Microplates||1 unit||2 units|
|Permeabilizing Buffer||1 x 30ml||2 x 30ml|
|Stop Solution||1 x 6ml||1 x 12ml|
Our Abpromise guarantee covers the use of ab115046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115046 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"