Overview

  • Product nameHistone H3 (methyl K4) Assay Kit (In Situ)
    See all Histone H3 (K4 methylation) kits
  • Sample type
    Adherent cells
  • Assay typeCell-based (quantitative)
  • Assay time
    3h 00m
  • Product overview

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.

    Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046) allows the user to specifically measure histone H3K4 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 10ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 100 µg/mL 1 x 60µl 1 x 120µl
    Detection Antibody, 200 µg/mL 1 x 20µl 1 x 40µl
    Developing Solution 1 x 12ml 1 x 24ml
    Methylated H3K4 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab115046 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046)

    ab115046 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"