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Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.
Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047) allows the user to specifically measure histone H3K9me in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
|Components||96 tests||2 x 96 tests|
|10X Wash Buffer||1 x 30ml||2 x 30ml|
|30% H2O2 Solution||1 x 0.5ml||1 x 1ml|
|8-Well Control Strips||2 units||4 units|
|Antibody Buffer||1 x 15ml||1 x 20ml|
|Blocking Buffer||1 x 20ml||2 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 60µl||1 x 120µl|
|Detection Antibody, 400 µg/mL||10 x 20µl||1 x 40µl|
|Developing Solution||1 x 12ml||1 x 24ml|
|Methylated H3K9 Control, 20 µg/mL||1 x 15µl||1 x 30µl|
|Microplates||1 unit||2 units|
|Permeabilizing Buffer||1 x 30ml||2 x 30ml|
|Stop Solution||1 x 6ml||1 x 12ml|
Our Abpromise guarantee covers the use of ab115047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115047 has not yet been referenced specifically in any publications.