Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)

Overview

  • Product name
    Histone H3 Modification Multiplex Assay Kit (Colorimetric)
    See all Histone H3 kits
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    0.5 ng/well
  • Range
    20 ng/well - 500 ng/well
  • Assay time
    2h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Plants, Fungi, Bacteria
  • Product overview

    The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab185910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

Protocols

References

This product has been referenced in:
  • Lillico R  et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). Read more (PubMed: 26721445) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
We used this product to detect histone modifications on bovine granulosa cells. It worked really well and gave good
signal. The background was very low even with brief washings.

Protocol:
1. Histone from bovine granulosa cells were extracted using histone extraction kit (ab113476)
2. Protein concentration in lysate was then measured using Bradford assay (BioRad)
3. 50 ng of histone extract was used per well in H3 modification multiplex assay kit (ab185910)
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Verified customer

Submitted Dec 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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