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Western blot
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ChIP - Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
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Immunocytochemistry/ Immunofluorescence - Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
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Product Name
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Histone H3 (mono methyl K4) antibody - ChIP Grade
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See all Histone H3 (methylated) antibodies (36)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 (mono methyl K4) - ChIP Grade
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, mono methylated at K4.
(Peptide available as ab1340.)
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Reacts with
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Hu, Cow, InMtj, Pig, Plnts, Tet, Xl
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Predicted to react
(species key)
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Mamm
(Due to sequence homology)
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Specificity
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Specific for mono-methylated Lysine 4 of histone H3.
Does not recognise di- or tri-methyl Lysine 4 nor methylation at Lysine 9.
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Tested applications
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ChIP, ChIP/Chip, Flow Cyt, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 2µg for 25µg of chromatin
ChIP/Chip: Use at an assay dependent dilution.
Flow Cyt: 1/100.
ICC/IF: Use at an assay dependent dilution.
IF: 1/500.
IHC-P: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). Can be blocked with Histone Histone H3 peptide - mono methyl K4 (ab1340).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Methylated
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Relevance
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Histones are basic nuclear proteins that, together with DNA, make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer, which is composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin structure is further compacted through the interaction of a linker histone H1.
Methylation of specific residues in the histone tails is a central modification for regulating epigenetic transitions in chromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, whilst arginine residues can be mono- or di-methylated.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.70 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 (mono methyl K4) antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 (mono methyl K4) antibody - ChIP Grade images, references, products related to ab8895 and other tools.
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Histone H3 (mono methyl K4) antibody - ChIP Grade images:
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 ChIP - Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab8895 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
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Immunocytochemistry/ Immunofluorescence - Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
Indian muntjac fibroblast cells - interphase (top left) and prophase (top right and below), stained with:
Mono Methyl K4 antibody, ab8895, (green)
DAPI: red, top left and right; blue, below
Phospho Ser 10 antibody: red (below) and blue (top right)
The perinuclear and perinucleolar heterochromatin domains do not contain Mono Methyl K4. The Mono Methyl K4, rather, is distributed as small nuclear foci primarily found between DAPI-intense regions of the nucleus.
Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta
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Flow Cytometry - Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
ab8895 staining mouse embryonic stem cells by flow cytometry. The ES cell colonies were trypsinized and the cells permeabilized before being stained with ab8895 (0.25ug/1.5 x 105 cells). A PE conjugated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an Abreview submitted by Prof Albrecht Müller
See Abreview
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References for Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
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This product has been used in: (two most recent references)
Heintzman ND et al. Histone modifications at human enhancers reflect global cell-type-specific gene expression. Nature 459:108-12 (2009). ChIP; Human. PubMed: 19295514
Gargiulo G et al. NA-Seq: a discovery tool for the analysis of chromatin structure and dynamics during differentiation. Dev Cell 16:466-81 (2009). ChIP. PubMed: 19289091
See all 56 publication references for this product.
If you publish research using ab8895 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Histone H3
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