Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric) (ab115055)

Overview

  • Product name
    Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric)
    See all Histone H3 (K4 methylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. H3 (mono-methyl K4) is associated with silenced euchromatin regions in the genome. H3 (mono-methyl K4) seems to accumulate at the 3' end of active genes both in yeast and mammals.
    Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric) (ab115055) allows the user to specifically measure global mono-methylation of histone H3-K4 fluorometrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab115055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of mono-methyl H3K4 was measured using ab115055.

Protocols

References

ab115055 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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