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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. H3 (mono-methyl K4) is associated with silenced euchromatin regions in the genome. H3 (mono-methyl K4) seems to accumulate at the 3' end of active genes both in yeast and mammals.
Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric) (ab115055) allows the user to specifically measure global mono-methylation of histone H3-K4 fluorometrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X wash buffer||1 x 10ml||1 x 20ml|
|8 well sample strips (with frame)||4 units||9 units|
|8 well standard control strips||2 units||3 units|
|Antibody buffer||1 x 6ml||1 x 12ml|
|Detection antibody, 1 mg/ml||1 x 5µl||1 x 10µl|
|Fluoro developer||1 x 12µl||1 x 24µl|
|Fluoro dilution||1 x 4ml||1 x 8ml|
|Fluoro enhancer||1 x 12µl||1 x 24µl|
|Standard control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of mono-methyl-H3-K4 was measured using ab115055.
ab115055 has not yet been referenced specifically in any publications.
Publishing research using ab115055 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"