Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084)

Overview

  • Product nameHistone H3 (mono-methyl K79) Quantification Kit (Fluorometric)See all Histone H3 (K79 methylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    0.4 ng/well
  • Range
    5 ng/well - 2000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fiber to stabilize interactions between enhancers and their target promoters.

    Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084) allows the user to specifically measure global mono-methylation of histone H3K79 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

     

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115084 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084)

    ab115084 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"