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Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fiber to stabilize interactions between enhancers and their target promoters.
Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084) allows the user to specifically measure global mono-methylation of histone H3K79 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115084 has not yet been referenced specifically in any publications.