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Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. H3 (mono-methyl K27), a modification enriched at periocentromic heterochromatin, is broadly distributed throughout all euchromatic sites and participates in silencing gene expression. H3 (di-methyl K27) and H3 (tri-methyl K27) are the facultative heterochromatin mark which promotes the recruitment of PcG proteins for gene silencing.
Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|10X Wash Buffer||1 x 20ml|
|8-Well Sample Strips (with Frame)||1 x 9 units|
|8-Well Standard Control Strips||Green Ringed||1 x 3 units|
|Antibody Buffer||1 x 12ml|
|Color Developer||1 x 10ml|
|Detection Antibody, 1 mg/mL||1 x 10µl|
|Standard Control, 100 µg/mL||1 x 20µl|
|Stop Solution||1 x 6ml|
Our Abpromise guarantee covers the use of ab115074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115074 has not yet been referenced specifically in any publications.