Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074)

Overview

  • Product nameHistone H3 (pan-methyl K27) Quantification Kit (Colorimetric)
    See all Histone H3 (K27 methylation) kits
  • Tests
    1 x 96 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. H3 (mono-methyl K27), a modification enriched at periocentromic heterochromatin, is broadly distributed throughout all euchromatic sites and participates in silencing gene expression. H3 (di-methyl K27) and H3 (tri-methyl K27) are the facultative heterochromatin mark which promotes the recruitment of PcG proteins for gene silencing.

    Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115074 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074)

    ab115074 has not yet been referenced specifically in any publications.

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