Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. H3 (mono-methyl K27), a modification enriched at periocentromic heterochromatin, is broadly distributed throughout all euchromatic sites and participates in silencing gene expression. H3 (di-methyl K27) and H3 (tri-methyl K27) are the facultative heterochromatin mark which promotes the recruitment of PcG proteins for gene silencing.
ab131560 allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K27 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
|10X Wash Buffer||1 x 20ml|
|8-Well Assay Strips (with Frame)||12 units|
|8-Well Standard Control Strips||Green Ringed||3 units|
|Antibody Buffer||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 10µl|
|Fluoro Developer||1 x 24µl|
|Fluoro Dilution||1 x 8ml|
|Fluoro Enhancer||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 20µl|
Our Abpromise guarantee covers the use of ab131560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab131560 has not yet been referenced specifically in any publications.