Histone H3 (pan-methyl K36) Quantification Kit (Colorimetric) (ab115081)

Overview

  • Product nameHistone H3 (pan-methyl K36) Quantification Kit (Colorimetric)
    See all Histone H3 (K36 methylation) kits
  • Tests
    1 x 96 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes. However, when present within protein-coding regions it prevents inappropriate transcriptional initiation of intragenic sequences. H3 (mono-methyl K36) is commonly found together with H3 (di-methyl K27) and associated with transcriptionally silenced genes. However, H3 (di-methyl K36) and H3 (tri-methyl K36) are generally associated with transcriptionally active genes.

    Histone H3 (pan-methyl K36) Quantification Kit (Colorimetric) (ab115081) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K36 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115081 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (pan-methyl K36) Quantification Kit (Colorimetric) (ab115081)

    ab115081 has not yet been referenced specifically in any publications.

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