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Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes. However, when present within protein-coding regions it prevents inappropriate transcriptional initiation of intragenic sequences. H3 (mono-methyl K36) is commonly found together with H3 (di-methyl K27) and associated with transcriptionally silenced genes. However, H3 (di-methyl K36) and H3 (tri-methyl K36) are generally associated with transcriptionally active genes.
Histone H3 (pan-methyl K36) Quantification Kit (Fluorometric) (ab115082) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K36 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|10X Wash Buffer||1 x 20ml|
|8-Well Sample Strips (with Frame)||1 x 9 units|
|8-Well Standard Control Strips||Green Ringed||1 x 3 units|
|Antibody Buffer||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 10µl|
|Fluoro Developer||1 x 24µl|
|Fluoro Dilution||1 x 8ml|
|Fluoro Enhancer||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 20µl|
Our Abpromise guarantee covers the use of ab115082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115082 has not yet been referenced specifically in any publications.