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Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. In mammals, H3(mono-methyl K4) is associated with silenced euchromatin regions whereas H3(di-methyl K4) and H3(tri-methyl K4) is associated with transcriptionally active euchromatin regions.
Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059) allows the user to specifically measure global mono-, di- and tri-methylation of histone H3K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
|10X Wash Buffer||1 x 20ml|
|8-Well Sample Strips (with Frame)||1 x 9 units|
|8-Well Standard Control Strips||1 x 3 units|
|Antibody Buffer||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 10µl|
|Fluoro Developer||1 x 24µl|
|Fluoro Dilution||1 x 8ml|
|Fluoro Enhancer||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 20µl|
Our Abpromise guarantee covers the use of ab115059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115059 has not yet been referenced specifically in any publications.