Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)

Overview

  • Product nameHistone H3 (pan-methyl K4) Quantification Kit (Fluorometric)
    See all Histone H3 (K4 methylation) kits
  • Tests
    1 x 96 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. In mammals, H3(mono-methyl K4) is associated with silenced euchromatin regions whereas H3(di-methyl K4) and H3(tri-methyl K4) is associated with transcriptionally active euchromatin regions.

    Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059) allows the user to specifically measure global mono-, di- and tri-methylation of histone H3K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115059 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)

    ab115059 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"