Histone H3 (pan-methyl K79) Quantification Kit (Colorimetric) (ab115089)

Overview

  • Product nameHistone H3 (pan-methyl K79) Quantification Kit (Colorimetric)
    See all Histone H3 (K79 methylation) kits
  • Tests
    1 x 96 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    2 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fibre to stabilize interactions between enhancers and their target promoters. H3 (di-methyl K79) and H3 (tri-methyl K79) are also widespread histone modifications and are associated with transcriptionally active genes. Increased global H3 (methyl K79) is also found to be involved in some pathological processes such as leukemogenesis in humans.

    Histone H3 (pan-methyl K79) Quantification Kit (Colorimetric) (ab115089) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K79 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115089 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (pan-methyl K79) Quantification Kit (Colorimetric) (ab115089)

    ab115089 has not yet been referenced specifically in any publications.

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