Histone H3 (pan-methyl K9) Quantification Kit (Colorimetric) (ab115066)

Overview

  • Product name
    Histone H3 (pan-methyl K9) Quantification Kit (Colorimetric)
    See all Histone H3 (K9 methylation) kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    1 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. H3 (mono-methyl K9) is enriched in certain euchromatic domains, which have been postulated to be transcriptionally silent. H3 (di-methyl K9) and H3 (tri-methyl K9) mediate heterochromatin formation by forming a binding site for HP1 and also participate in silencing gene expression at euchromatic sites.
    Histone H3 (pan-methyl K9) Quantification Kit (Colorimetric) (ab115066) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab115066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References

ab115066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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