Histone H3 (pan-methyl K9) Quantification Kit (Colorimetric) (ab115066)

Overview

  • Product nameHistone H3 (pan-methyl K9) Quantification Kit (Colorimetric)
    See all Histone H3 (K9 methylation) kits
  • Tests
    1 x 96 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Sensitivity
    1 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. H3 (mono-methyl K9) is enriched in certain euchromatic domains, which have been postulated to be transcriptionally silent. H3 (di-methyl K9) and H3 (tri-methyl K9) mediate heterochromatin formation by forming a binding site for HP1 and also participate in silencing gene expression at euchromatic sites.
    Histone H3 (pan-methyl K9) Quantification Kit (Colorimetric) (ab115066) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115066 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H3 (pan-methyl K9) Quantification Kit (Colorimetric) (ab115066)

    ab115066 has not yet been referenced specifically in any publications.

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