Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. H3 (mono-methyl K9) is enriched in certain euchromatic domains, which have been postulated to be transcriptionally silent. H3 (di-methyl K9) and H3 (tri-methyl K9) mediate heterochromatin formation by forming a binding site for HP1 and also participate in silencing gene expression at euchromatic sites.
Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K9 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|10X Wash Buffer||1 x 20ml|
|8-Well Sample Strips (with Frame)||1 x 9 units|
|8-Well Standard Control Strips||Green Ringed||1 x 3 units|
|Antibody Buffer||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 10µl|
|Fluoro Developer||1 x 24µl|
|Fluoro Dilution||1 x 8ml|
|Fluoro Enhancer||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 20µl|
Our Abpromise guarantee covers the use of ab115067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115067 used to specifically measure global histone H3K9 mono-, di-, and tri-methylation. The ratio of mono-, di-, and tri-methylated H3K9 is proportional to the intensity of fluorescence. The absolute amount of the methylated H3K9 can be quantitated by comparing to the standard control.
ab115067 has not yet been referenced specifically in any publications.