RabMAb

Anti-Histone H3 (phospho S10) antibody [E173] (ab32107)

Overview

  • Product nameAnti-Histone H3 (phospho S10) antibody [E173]See all Histone H3 primary antibodies ...
  • Description
    Rabbit monoclonal [E173] to Histone H3 (phospho S10)
  • SpecificityThis antibody is specific for Histone H3 phosphorylated on Serine 10 only.
  • Tested applicationsICC/IF, WB, IHC-P, ICC, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Fruit fly (Drosophila melanogaster)
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic phospho peptide corresponding to residues surrounding Serine 10 of Human Histone H3.

  • Positive control
    • WB: Hela cell lysate. IHC-P: Lymphoma tissue.
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 or Alexa Fluor® 488 see ab139848 or ab154206 respectively.

    A trial size is available for this product.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • Clonality Monoclonal
  • Clone numberE173
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab32107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/1000. See Abreview 20th Feb 2007.
WB 1/2500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P Use at an assay dependent dilution.
ICC 1/250.
IP 1/40.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionVariant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Sequence similaritiesBelongs to the histone H3 family.
    • Developmental stageExpressed throughout the cell cycle independently of DNA synthesis.
    • Post-translational
      modifications
      Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin. Phosphorylation on Ser-32 (H3S31ph) is specific to regions bordering centromeres in metaphase chromosomes.
      Ubiquitinated. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination.
    • Cellular localizationNucleus. Chromosome.
    • Target information above from: UniProt accession P84243 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
      • H3 3 like sequence MH921 antibody
      • H3 3A antibody
      • H3 a antibody
      • H3 b antibody
      • H3 c antibody
      • H3 d antibody
      • H3 f antibody
      • H3 h antibody
      • H3 histone family member E pseudogene antibody
      • H3 i antibody
      • H3 j antibody
      • H3 k antibody
      • H3 l antibody
      • H33_HUMAN antibody
      • H3F3 antibody
      • H3f3b antibody
      • Histone H3 3 pseudogene antibody
      • Histone H3.3 antibody
      see all

    Anti-Histone H3 (phospho S10) antibody [E173] images

    • All lanes : Anti-Histone H3 (phospho S10) antibody [E173] (ab32107) at 1/2500 dilution

      Lane 1 : Hela cell lysate, untreated.
      Lane 2 : Hela cell lysate, treated with Alkaline Phosphatase.


      Predicted band size : 15 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Ab32107, at a 1/50 dilution, staining Histone H3 in paraffin embedded lymphoma tissue by Immunohistochemistry.
    • ab32107 (1/1000) staining Histone H3 (phospho S10) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody: Goat anti-Rabbit IgG conjugated to Cy3® (1/200). Please refer to abreview for further details.

      See Abreview

    References for Anti-Histone H3 (phospho S10) antibody [E173] (ab32107)

    This product has been referenced in:
    • Hasegawa K  et al. Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells. J Cell Biol 200:151-61 (2013). WB ; Human . Read more (PubMed: 23319601) »
    • Mo QQ  et al. Inhibition of Hec1 expression enhances the sensitivity of human ovarian cancer cells to paclitaxel. Acta Pharmacol Sin 34:541-8 (2013). Human . Read more (PubMed: 23474708) »

    See all 10 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
    Specification 0-4hr embryos
    Treatment control shRNA and wee shRNA embryos
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Richelle Sopko

    Verified customer

    Submitted Dec 04 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Feb 20 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"