Histone H3 (phospho S10) Assay Kit (In Situ) (ab115126)

Overview

  • Product nameHistone H3 (phospho S10) Assay Kit (In Situ)
    See all Histone H3 (S10 Phosphorylation) kits
  • Tests
    1 x 96 test
  • Sample type
    Adherent cells
  • Assay typeCell-based (quantitative)
  • Assay time
    3h 00m
  • Species reactivity

    Predicted to work with: all Mammals
  • Product overview

    Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. Detection in the change of histone H3 (phospho S10) associated with the cell cycle, apoptosis, inhibitor or activator treatment would provide useful information for better understanding the pathological process of some diseases.

    Abcam's Histone H3 (phospho S10) Assay Kit (In Situ) (ab115126) allows the user to measure histone H3 (phospho S10) in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115126 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (phospho S10) Assay Kit (In Situ) images

    • Log-phase MCF-7 cells were serum-starved overnight, and serum was added back for 30 minutes followed by treatment with Calyculin A (CL-A, 0.2 µM for 30 minutes). The level of H3 phosphorylation at ser10 was measured with ab115126.

    Protocols

    References for Histone H3 (phospho S10) Assay Kit (In Situ) (ab115126)

    ab115126 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115126.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"