• Product nameAnti-Histone H3 (phospho S28) antibody
    See all Histone H3 primary antibodies
  • Description
    Rabbit polyclonal to Histone H3 (phospho S28)
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Drosophila melanogaster
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (phospho S28) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P68431
    (Peptide available as ab14793)

  • Positive control
    • Colcemid-treated HeLa histone Preparation; Hela whole cell extract


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab4178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa.Can be blocked with Human Histone H3 (phospho S28) peptide (ab14793).
ICC/IF 1/50.


  • FunctionVariant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H3 family.
  • Developmental stageExpressed throughout the cell cycle independently of DNA synthesis.
  • Post-translational
    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin. Phosphorylation on Ser-32 (H3S31ph) is specific to regions bordering centromeres in metaphase chromosomes.
    Ubiquitinated. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 a antibody
    • H3 b antibody
    • H3 c antibody
    • H3 d antibody
    • H3 f antibody
    • H3 h antibody
    • H3 histone family member E pseudogene antibody
    • H3 i antibody
    • H3 j antibody
    • H3 k antibody
    • H3 l antibody
    • H33_HUMAN antibody
    • H3F3 antibody
    • H3f3b antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.3 antibody
    see all

Anti-Histone H3 (phospho S28) antibody images

  • This image was kindly supplied as part of the review submitted by Monika Harrer.
  • All lanes : Anti-Histone H3 (phospho S28) antibody (ab4178)

    Lane 1 : Calf thymus histone lysate
    Lane 2 : colecemid-treated histones at 10 µg
    Lane 3 : Calf thymus histone lysate with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml
    Lane 4 : colecemid-treated histones at 10 µg with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml
    Lane 5 : Calf thymus histone lysate with Human Histone H3 (unmodified ) peptide (ab14794) at 1 µg/ml
    Lane 6 : colecemid-treated histones at 10 µg with Human Histone H3 (unmodified ) peptide (ab14794) at 1 µg/ml

    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 15.4 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    Rabbit polyclonal to Histone H3 (phospho S28) (ab4178) at 1/500. Each lane contains 10ug of histone prep (HeLa cell acid extract).

    Lane 1 : Histone prep
    Lane 2 : Colecemid-treated histones
    Lane 3 : Histone prep + 1ug/ml of phospho S28 blocking peptide
    Lane 4 : Colecemid-treated histones + 1ug/ml of phospho S28 blocking peptide
    Lane 5 : Histone prep + 1ug/ml of non-phospho S28 blocking peptide
    Lane 6 : Colecemid-treated histones + 1ug/ml of non-phospho S28 blocking peptide

    Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.

  • All lanes : Anti-Histone H3 (phospho S28) antibody (ab4178) at 1/1000 dilution

    Lane 1 : control shRNA expresssing 0-4 hour old Drosophila embryos
    Lane 2 : wee shRNA expresssing 0-4 hour old Drosophila embryos

    Donkey anti-rabbit IgG, Horseradish Peroxidase-L at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15.4 kDa

    Exposure time : 2 seconds

    Image courtesy of Richelle Sopko, Harvard University, U.S.A.

    Blocking: 10% BSA

    wee shRNA embryos (lane 2) should display elevated phospho H3Ser28 levels relative to 0-4hr old EGFP shRNA embryos (lane 1)

    See Abreview

References for Anti-Histone H3 (phospho S28) antibody (ab4178)

This product has been referenced in:
  • Shimada M  et al. cAMP-response element-binding protein (CREB) controls MSK1-mediated phosphorylation of histone H3 at the c-fos promoter in vitro. J Biol Chem 285:9390-401 (2010). WB ; Human . Read more (PubMed: 20089855) »
  • Coxon CH  et al. Over expression of Plk1 does not induce cell division in rat cardiac myocytes in vitro. PLoS One 4:e6752 (2009). Read more (PubMed: 19707596) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryo)
Specification 0-4hr embryo
Treatment control shRNA and wee shRNA embryos
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Richelle Sopko

Verified customer

Submitted Dec 18 2013