Phosphorylation of histone H3 at serine 28 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. In vitro studies have shown that phosphorylation of histone H3 at both S10 and S28 is coupled with dynamic acetylation of H3, where histone H3 (phospho S28) had a higher steady state of acetylation than that of H3 (phospho S10). H3 (phospho S28) is regulated by the cell cycle and has been used as a mitotic marker.
Abcam's Histone H3 (phospho S28) Assay Kit (Fluorometric) (ab115130) allows the user to measure global histone H3 (phospho S28) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser28 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8 Well Sample Strips (with Frame)||4 units||9 units|
|8 Well Standard Control Strips||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115130 has not yet been referenced specifically in any publications.