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Our Abpromise guarantee covers the use of ab14102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H3 (phospho T6) peptide (ab14103).|
|PepArr||Use a concentration of 0.2 - 0.02 µg/ml.|
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
All batches of ab14102 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - phospho T6 peptide (ab14103), indicating that this antibody specifically recognises the Histone H3 - phospho T6 modification.
ab7228 - Histone H3 unmodified
ab17578 - Histone H3 - phospho T3
ab14103 - Histone H3 - phospho T6
ab24444 - Histone H3 - phospho T11
ab11477 - Histone H3 - phospho S10
ab32876 - Histone H3 - citrulline 2 + 8 + 17
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"