Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)

Overview

  • Product nameHistone H3 Total Acetylation Detection Fast Kit (Colorimetric)
    See all Histone H3 acetylation kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    2 ng/well
  • Range
    5 ng/well - 2000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
    Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115124 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) images

    • Acetylation Histone 3 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 20-500 ng per well) (duplicates +/- SD).

    • Standard curve with background signal subtracted (duplicates; +/- SD).

    Protocols

    References for Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)

    This product has been referenced in:
    • Kocic G  et al. Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease. Ren Fail 36:1078-82 (2014). Functional Studies ; Human . Read more (PubMed: 24845033) »
    • Dong G  et al. Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows. PLoS One 9:e107659 (2014). Functional Studies ; Cow . Read more (PubMed: 25222274) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    This assay was performed on histones isolated from the flesh fly, Sarcophaga bullata. Histones were isolated from diapause and control pupae using the Histone Extraction kit (ab113476) and had been stored at -80 °C for approximately 6 months before being used in the assay. The total protein for each sample was estimated by measuring absorbance at 280 nm with a nanodrop spectrophotometer. All samples were diluted to 1 µg of protein using the Antibody buffer provided with the kit. A range of protein amounts (1-10 µg) were used in the assay.
    The assay was conducted according to the provided instructions. Samples and standards were incubated at 25 °C for ~ 100 minutes before proceeding with the first wash step. Average, blank corrected, O.D. values for acetylated standards ranged from 0.221 to 5.86 the curve was approximately logarithmic (see below). The average, blank corrected, O.D. values ranged from 0.014 to 0.1 for control samples and 0.006 to 0.283 for diapause samples. Only the 10 µg per well diapause samples had O.D. values within the range of the standards. The control samples appeared to have ~ 50 % less acetylated histone protein than the diapause samples (see figure below), as was expected from our previous research.
    In summary, the kit was easy to use and does appear to work with our non-model insect.
    Username

    Dr. Julie Reynolds

    Verified customer

    Submitted Feb 08 2016



    The detection antibody in ab115124 kit is labeled with signal reporter already. But H4 antibody included in the kit ab115125 is not and needs to be labeled by signal reporter and enhanced with enhancer during the assay.



    1. Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

    2. The minimum amount of histone protein required as input for these assays are 50 ng (both for the colorimetric and fluorometric kits...

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    Compared to ab115102, ab115124 is much faster and easier to handle with higher sensitivity and better reproducibility, but slightly less specificity. 1-2 ug of histone protein (min 50,000 cells) may be needed for each assay point if using ab115102, whi...

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    Thank you for contacting us. We have not tested these kits in fish and have no testing data on this use. The kit ab115103 may be suitable for use in fish based on principle. However, the kit ab115124 may not be suitable for fish.

    I hope this...

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    The kits are very similar. The main differences are that the ab115102 requires 5 hrs to run and you need to coat the antibody yourself while the kit ab115124 only requires 2.5 hours and the wells come pre-coated.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"