Histone H3 Total Quantification Kit (Colorimetric) (ab115091)

Overview

  • Product name
    Histone H3 Total Quantification Kit (Colorimetric)
    See all Histone H3 kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    10 ng/well
  • Range
    20 ng/well - 1000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammal
  • Product overview

    Histone H3 Total Quantification Kit (Colorimetric) (ab115091) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.


    Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab115091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Measurement of histone H3 with different concentrations of the included H3 standard in theHistone H3 Total Quantification Kit (Colorimetric) (ab115091).

Protocols

References

ab115091 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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