Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.
Histone H3 Total Quantification Kit (Fluorometric) (ab115092) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8 well Sample Strips (with frame)||4 units||9 units|
|8 well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|Standard Control (100 µg/mL)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115092 has not yet been referenced specifically in any publications.