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Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, tri-methylation of histone H3 at lysine 27 (H3 (tri-methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri-methyl K27) is also found to be involved in some pathological processes such as cancer progression.
Abcam's Histone H3 (tri-methyl K27) Assay Kit (ab115051) is suitable for specifically measuring global histone H3K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Lysis Buffer||1 x 5ml||1 x 10ml|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Detection Antibody, 400 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 5ml||1 x 10ml|
|Extraction Buffer||1 x 8ml||1 x 16ml|
|H3K27me3 Control, 60 µg/mL||1 x 10µl||1 x 20µl|
|Histone Buffer||1 x 0.5ml||1 x 1ml|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115051 has not yet been referenced specifically in any publications.