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Histone H3 (tri-methyl K27) Assay Kit (ab115051) is suitable for specifically measuring global histone H3K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, tri-methylation of histone H3 at lysine 27 (H3 (tri-methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri-methyl K27) is also found to be involved in some pathological processes such as cancer progression.
|Components||48 tests||96 tests|
|10X Lysis Buffer||1 x 5ml||1 x 10ml|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Detection Antibody, 400 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 5ml||1 x 10ml|
|Extraction Buffer||1 x 8ml||1 x 16ml|
|H3K27me3 Control, 60 µg/mL||1 x 10µl||1 x 20µl|
|Histone Buffer||1 x 0.5ml||1 x 1ml|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115051 has not yet been referenced specifically in any publications.