Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045)

Overview

  • Product name
    Histone H3 (tri methyl K27) Assay Kit (In Situ)
    See all Histone H3 (K27 methylation) kits
  • Sample type
    Adherent cells
  • Assay type
    Cell-based (quantitative)
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, tri-methylation of histone H3 at lysine 27 (H3 (tri methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.

    Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045) allows the user to specifically measure histone H3K27me3 in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 10ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 1000 µg/mL 1 x 9µl 1 x 18µl
    Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl
    Developing Solution 1 x 12ml 1 x 24ml
    H3K27me3 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplate 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab115045 detecting Histone H3 (tri methyl K27) in MDA-231 cancer cells. Cells were grown in microplate wells for 48 hours.

Protocols

References

ab115045 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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