Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, tri-methylation of histone H3 at lysine 27 (H3 (tri methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045) allows the user to specifically measure histone H3K27me3 in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
|Components||96 tests||2 x 96 tests|
|10X Wash Buffer||1 x 30ml||2 x 30ml|
|30% H2O2 Solution||1 x 0.5ml||1 x 1ml|
|8-Well Control Strips||2 units||4 units|
|Antibody Buffer||1 x 10ml||1 x 20ml|
|Blocking Buffer||1 x 20ml||2 x 20ml|
|Capture Antibody, 1000 µg/mL||1 x 9µl||1 x 18µl|
|Detection Antibody, 200 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 12ml||1 x 24ml|
|H3K27me3 Control, 20 µg/mL||1 x 15µl||1 x 30µl|
|Microplate||1 unit||2 units|
|Permeabilizing Buffer||1 x 30ml||2 x 30ml|
|Stop Solution||1 x 6ml||1 x 12ml|
Our Abpromise guarantee covers the use of ab115045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115045 has not yet been referenced specifically in any publications.