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Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, H3(try methyl K27) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
Histone H3 (tri-methyl K27) Quantification Kit (Fluorometric) (ab115073) allows the user to specifically measure global tri-methylation of histone H3K27 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro-Developer||1 x 12µl||1 x 24µl|
|Fluoro-Dilution||1 x 4ml||1 x 8ml|
|Fluoro-Enhancer||1 x 12µl||1 x 24µl|
|Standard Control (100 µg/mL)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"