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Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. Enrichment of H3 (tri-methyl K4) is found at transcription start sites of active genes where they contribute to transcriptional initiation.
Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with frame)||4 units||9 units|
|8-Well Standard Control Strips||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Color Developer||1 x 5ml||1 x 10ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Standard Control (100 µg/ml)||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K4 was measured using ab115056.
ab115056 has not yet been referenced specifically in any publications.