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ChIP - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)
![ChIP - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/ps/datasheet/images/ab71604_3.jpg)
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Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)
![Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/ps/datasheet/images/71/ab71604/Histone-H3-Primary-antibodies-ab71604-5.jpg)
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Western blot - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)
![Western blot - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/ps/datasheet/images/71/ab71604/Histone-H3-Primary-antibodies-ab71604-8.jpg)
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Product Name
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Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade
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See all Histone H3 (methylated) antibodies (36)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [6F12-H4] to Histone H3 (tri methyl K9, di methyl K9) - ChIP Grade
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Immunogen
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Synthetic peptide of Human Histone H3, tri methylated at K9.
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Reacts with
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Hu, Cow
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Predicted to react
(species key)
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Ms, Rat, Many, Dm, Hm, NHuPrm, Pig, Rb, Sc
(Due to sequence homology)
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Specificity
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In Western blot ab71604 is blocked by tri methyl K9 and di methyl K9 peptides. It is not blocked by non-modified peptides or by methylated K4 or K27 peptides.
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Tested applications
(see key)
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ChIP, ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 2 - 5 µg for 25 µg of chromatin
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). Can be blocked with Histone H3 peptide - tri methyl K9 (ab1773).
ELISA: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in a calf thymus histone preparation.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Methylated
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Relevance
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Histones are basic nuclear proteins that, together with DNA, make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer, which is composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin structure is further compacted through the interaction of a linker histone H1.
Methylation of specific residues in the histone tails is a central modification for regulating epigenetic transitions in chromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, whilst arginine residues can be mono- or di-methylated.
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Alternative names Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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6F12-H4
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Myeloma
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x63-Ag8.653
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.70 - 0.70 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade images, references, products related to ab71604 and other tools.
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Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade images:
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 ChIP - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab71604 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)
ICC/IF image of ab71604 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71604, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, MCF7 and HepG2 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
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ELISA - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)
![ELISA - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/ps/datasheet/images/71/ab71604/Histone-H3-Primary-antibodies-ab71604-10.jpg)
ELISA using ab71604 at varying antibody concentrations. Curve_SPL7 indicates binding to the tri methyl K9 peptide ab1773. In addition, SPL6 indicates binding to the di methyl K9 peptide ab1772. Binding to the following peptides was not seen:
SPL1 unmodified Histone H3, SPL2 Histone H3 mono methyl K4, SPL3 Histone H3 di methyl K4, SPL4 Histone H3 tri methyl K4, SPL5 Histone H3 mono methyl K9, SPL8 Histone H3 mono methyl K27, SPL9 Histone H3 di methyl K27, SPL10 Histone H3 tri methyl K27.
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Search PubMed (MEDLINE) for references to Histone H3
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Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![ChIP - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/index.html?datasheet=71604#ab71604_3.jpg)
![Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/index.html?datasheet=71604#Histone-H3-Primary-antibodies-ab71604-5.jpg)
![Western blot - Histone H3 (tri methyl K9, di methyl K9) antibody [6F12-H4] - ChIP Grade (ab71604)](/index.html?datasheet=71604#Histone-H3-Primary-antibodies-ab71604-8.jpg)
