Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. In particular, H3(tri -methyl K9) mediates heterochromatin formation by forming a binding site for HP and is a stable heterochromatin mark which promotes gene silencing.is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing.
Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (ab115064) allows the user to specifically measure global tri-methylation of histone H3K9 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Color Developer||1 x 5ml||1 x 10ml|
|Detection Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Standard Control (100 µg/mL)||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K9 was measured using ab115064.