Overview

  • Product name
    Anti-Histone H4 (acetyl K12) antibody
    See all Histone H4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H4 (acetyl K12)
  • Specificity
    ab61238 detects endogenous levels of Histone H4 only when acetylated at lysine 12.
  • Tested applications
    Suitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Drosophila melanogaster
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 10-15 (acetyl K12). Synthetic acetylated peptide derived from human Histone H4 around the acetylation site of lysine 12

    LGK(Ac)GG


    Sequence:

    LGK(Ac)GG


    Database link: P62805

  • Positive control
    • Human breast carcinoma tissue (IHC-P). Extracts of COS7 cells treated with TSA (400nM, 24hours) (WB).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ICC/IF 1/500 - 1/1000.

Target

Anti-Histone H4 (acetyl K12) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K12) antibody (ab61238) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.
  • All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, treated with TSA (400nM, 24hours) with no acetylated peptide
    Lane 2 : Extracts from COS7 cells, treated with TSA (400nM, 24hours) with acetylated peptide


    Predicted band size : 11 kDa
    Observed band size : 11 kDa

References for Anti-Histone H4 (acetyl K12) antibody (ab61238)

This product has been referenced in:
  • Zhao M  et al. Ketogenic diet improves the spatial memory impairment caused by exposure to hypobaric hypoxia through increased acetylation of histones in rats. PLoS One 12:e0174477 (2017). WB ; Rat . Read more (PubMed: 28355243) »
  • Suzuki T  et al. Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes. Nat Commun 7:12676 (2016). IHC (PFA fixed) ; Mouse . Read more (PubMed: 27623537) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Purified protein (human hepatocarcimona, HepG2)
Specification
human hepatocarcimona, HepG2
Treatment
Various media condition for 24hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Chanhee Jo

Verified customer

Submitted Dec 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Oocyte)
Specification
Oocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Username

Dr. Maki Asami

Verified customer

Submitted Oct 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount
20 µg
Specification
Head
Gel Running Conditions
Reduced Denaturing (gel %12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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