Use at an assay dependent concentration. 2 - 8 ul per IP
Use at an assay dependent concentration.
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
Post-translational modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression.
ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Western blot - Histone H3 (acetyl K12) antibody (ab46983)
All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution
Lane 1 : Acid extract of
HeLa cells without treatment. Lane 2 : Acid extract of
HeLa cells treated
with sodium butyrate.
Predicted band size : 11 kDa Observed band size : 11 kDa
Dot Blot - Anti-Histone H4 (acetyl K12) antibody (ab46983)
ab46983 in dot blot (top panel), acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1000. The amount of peptide (picomoles) spotted is shown next to each row.
Lane 1: Histone H4 acetyl-Lys5 peptide. Lane 2: Unmodified Lys5 peptide. Lane 3: Acetyl-Lys8 peptide. Lane 4: Unmodified Lys8 peptide. Lane 5: Acetyl-Lys12 peptide. Lane 6: Unmodified Lys12 peptide. Lane 7: Acetyl-Lys16 peptide. Lane 8: Unmodified Lys16 peptide.
ab46983 in WB (bottom panel)at 1/2000 with whole-cell extracts (35 ug/lane)from wildtype yeast or yeast containing a mutated histone H4 gene. Lane 1: Wild-type H4. Lane 2: H4 N-terminal tail deleted. Lane 3: Histone H4 K5 to R. Lane 4: H4 with K8 to R. Lane 5: H4 with K12 to R. Lane 6: H4 K16 to R. Lane 7: H4 K5, 8 and 12 to R.
Sun J et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun6:8856 (2015).
IHC (PFA fixed)
Read more (PubMed: 26581759) »
Intlekofer KA et al. Exercise and Sodium Butyrate Transform a Subthreshold Learning Event into Long-Term Memory via a Brain-Derived Neurotrophic factor-Dependent Mechanism. NeuropsychopharmacologyN/A:N/A (2013).
Read more (PubMed: 23615664) »