Use at an assay dependent concentration. 2 - 8 ul per IP
Use at an assay dependent concentration.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Belongs to the histone H4 family.
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression.
ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).
Western blot - Histone H3 (acetyl K12) antibody (ab46983)
All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution
Lane 1 : Acid extract of
HeLa cells without treatment. Lane 2 : Acid extract of
HeLa cells treated
with sodium butyrate.
Predicted band size : 11 kDa Observed band size : 11 kDa
Dot Blot - Anti-Histone H4 (acetyl K12) antibody (ab46983)
ab46983 in dot blot (top panel), acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1000. The amount of peptide (picomoles) spotted is shown next to each row.
Lane 1: Histone H4 acetyl-Lys5 peptide. Lane 2: Unmodified Lys5 peptide. Lane 3: Acetyl-Lys8 peptide. Lane 4: Unmodified Lys8 peptide. Lane 5: Acetyl-Lys12 peptide. Lane 6: Unmodified Lys12 peptide. Lane 7: Acetyl-Lys16 peptide. Lane 8: Unmodified Lys16 peptide.
ab46983 in WB (bottom panel)at 1/2000 with whole-cell extracts (35 ug/lane)from wildtype yeast or yeast containing a mutated histone H4 gene. Lane 1: Wild-type H4. Lane 2: H4 N-terminal tail deleted. Lane 3: Histone H4 K5 to R. Lane 4: H4 with K8 to R. Lane 5: H4 with K12 to R. Lane 6: H4 K16 to R. Lane 7: H4 K5, 8 and 12 to R.
Sun J et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun6:8856 (2015).
IHC (PFA fixed)
Read more (PubMed: 26581759) »
Intlekofer KA et al. Exercise and Sodium Butyrate Transform a Subthreshold Learning Event into Long-Term Memory via a Brain-Derived Neurotrophic factor-Dependent Mechanism. NeuropsychopharmacologyN/A:N/A (2013).
Read more (PubMed: 23615664) »