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Products:Epigenetics and Nuclear Signaling >> Histones >> H4 >> Acetylated
Overview
Product name
Anti-Histone H4 (acetyl K16) antibody - ChIP Grade
See all Histone H4 products (13) ...
Description
Rabbit polyclonal to Histone H4 (acetyl K16) - ChIP Grade
Specificity
ab61240 detects endogenous levels of Histone H4 only when acetylated at lysine 16.
Tested applications
WB, ChIP, ICC/IF, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Saccharomyces cerevisiae
Immunogen
Synthetic acetylated peptide derived from human Histone H4 around the acetylation site of lysine 16 (GAKAcRH).
Positive control
Human breast carcinoma tissue (IHC-P). Jurkat cells (ICC/IF).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> H4 >> Acetylated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (PubMed: 21726436)
ChIP: Use at an assay dependent dilution. (PubMed: 19626119)
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similarities
Belongs to the histone H4 family.
Post-translational
modifications
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP62805
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H4 antibody
- H4_HUMAN antibody
- H4F2 antibody
see all
Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all
Anti-Histone H4 (acetyl K16) antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 (acetyl K16) antibody (ab61240)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K16) antibody (ab61240) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.
Immunocytochemistry/ Immunofluorescence - Histone H4 (acetyl K16) antibody (ab61240)
Immunofluorescence analysis of Jurkat cells, using Histone H4 (acetyl K16) antibody (ab61240) at 1/500 - 1/1000 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
References for Anti-Histone H4 (acetyl K16) antibody - ChIP Grade (ab61240)
This product has been referenced in:
- Vaquero-Sedas MIet al. Arabidopsis thaliana telomeres exhibit euchromatic features. Nucleic Acids Res 39:2007-17 (2011). ChIP; Arabidopsis thaliana.Read more (PubMed: 21071395) »
- Zhu Xet al. Mediator influences telomeric silencing and cellular life span. Mol Cell Biol 31:2413-21 (2011). ChIP.Read more (PubMed: 21482672) »
See all 6 publications for this product
Publishing research using ab61240? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"