Anti-Histone H4 (acetyl K5) antibody (ab124636)

Overview

  • Product nameAnti-Histone H4 (acetyl K5) antibody
    See all Histone H4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H4 (acetyl K5)
  • Specificityab124636 detects endogenous levels of total Histone H4 protein only when acetylated at Lysine 5.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (acetyl K5). A synthetic peptide from human Histone H4 around the acetylated site of Lys5.
    Database link: P62805

  • Positive control
    • Human prostate, COS7 cells, HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.9% Sodium chloride
    Note: without Mg2+, Ca2+
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab124636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H4 family.
  • Post-translational
    modifications
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Histone gene cluster 1, H4A antibody
    • Histone gene cluster 2, H4 antibody
    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4 histone family, member A antibody
    • H4 histone family, member B antibody
    • H4 histone family, member C antibody
    • H4 histone family, member D antibody
    • H4 histone family, member E antibody
    • H4 histone family, member G antibody
    • H4 histone family, member H antibody
    • H4 histone family, member I antibody
    • H4 histone family, member J antibody
    • H4 histone family, member K antibody
    • H4 histone family, member M antibody
    • H4 histone family, member N antibody
    • H4 histone, family 2 antibody
    • H4/A antibody
    • H4/B antibody
    • H4/C antibody
    • H4/D antibody
    • H4/E antibody
    • H4/G antibody
    • H4/H antibody
    • H4/I antibody
    • H4/J antibody
    • H4/K antibody
    • H4/M antibody
    • H4/N antibody
    • H4/O antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • HIST1 cluster, H4A antibody
    • HIST1 cluster, H4B antibody
    • HIST1 cluster, H4D antibody
    • HIST2H4 antibody
    • Hist4 cluster, H4 antibody
    • Hist4h4 antibody
    • histone 1, H4a antibody
    • histone 1, H4c antibody
    • histone 1, H4d antibody
    • histone 1, H4f antibody
    • histone 1, H4h antibody
    • histone 1, H4i antibody
    • histone 1, H4j antibody
    • histone 1, H4k antibody
    • histone 1, H4l antibody
    • histone 2, H4a antibody
    • histone 2, H4b antibody
    • Histone 4 family, member M antibody
    • histone 4, H4 antibody
    • histone cluster 1, H4 antibody
    • histone cluster 1, H4a antibody
    • histone cluster 1, H4b antibody
    • histone cluster 1, H4c antibody
    • histone cluster 1, H4d antibody
    • histone cluster 1, H4e antibody
    • histone cluster 1, H4f antibody
    • histone cluster 1, H4h antibody
    • histone cluster 1, H4i antibody
    • histone cluster 1, H4j antibody
    • histone cluster 1, H4k antibody
    • histone cluster 1, H4l antibody
    • histone cluster 2, H4a antibody
    • histone cluster 2, H4b antibody
    • histone cluster 4, H4 antibody
    • Histone family, member A antibody
    • Histone family, member B antibody
    • Histone family, member D antibody
    • Histone family, member H antibody
    • Histone family, member I antibody
    • Histone family, member L antibody
    • Histone gene cluster 1, H4 antibody
    • Histone gene cluster 1, H4D antibody
    • Histone gene cluster 1, H4E antibody
    • Histone gene cluster 1, H4K antibody
    • Histone gene cluster 4, H4 antibody
    • Histone gene cluster 4, H4 histone antibody
    • Histone H4 antibody
    • histone IV, family 2 antibody
    see all

Anti-Histone H4 (acetyl K5) antibody images

  • ab124636, at 5µg/mL, staining Human prostate by immunohistochemistry of paraffin embedded tissues (IHC-P).
  • ab124636, at 1/100 dilution, staining HeLa cells, by immunofluorescence. The picture on the right is treated with synthesized peptide.
  • All lanes : Anti-Histone H4 (acetyl K5) antibody (ab124636) at 1/500 dilution

    Lane 1 : COS7 cell extract, treated with TSA 400nM 24h.
    Lane 2 : COS7 cell extract, treated with TSA 400nM 24h. with synthesized peptide


    Predicted band size : 11 kDa

References for Anti-Histone H4 (acetyl K5) antibody (ab124636)

ab124636 has not yet been referenced specifically in any publications.

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