Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Histones >> H4 >> Acetylated
Overview
Product name
Anti-Histone H4 (acetyl K91) antibody - ChIP Grade
See all Histone H4 products (13) ...
Description
Rabbit polyclonal to Histone H4 (acetyl K91) - ChIP Grade
Tested applications
WB, CHIPseq, IP, ChIPmore details
Cross reactivity
Reacts with
Cow, Saccharomyces cerevisiae
Predicted to work with
Mouse, Rat, Chicken, Human, Pig, Xenopus laevis, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Duck
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Histone H4, acetylated at K91.
(Peptide available as ab10112.)
Positive control
Calf thymus histone prep.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> H4 >> Acetylated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 11 kDa (predicted molecular weight: 12 kDa).
CHIPseq: Use at an assay dependent dilution.
IP: 1/75
ChIP: Use at an assay dependent dilution.
Target
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similarities
Belongs to the histone H4 family.
Post-translational
modifications
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP62805
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H4 antibody
- H4_HUMAN antibody
- H4F2 antibody
see all
Database links
- Entrez Gene: 280691 Cow
- Entrez Gene: 318846 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 3771854 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 3771893 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 3771908 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 3771935 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 3771938 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 3771941 Fruit fly (Drosophila melanogaster)
see all
Anti-Histone H4 (acetyl K91) antibody - ChIP Grade images:
Western blot
Lanes 1 & 3 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/500 dilution
Lanes 2 & 4 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/1000 dilution
Lane 1 : Calf thymus histone prep.
Lane 2 : Calf thymus histone prep.
Lane 3 : Calf thymus histone prep. with Histone H4 peptide - acetyl K91 (ab10112) at 2 µg
Lane 4 : Calf thymus histone prep. with Histone H4 peptide - acetyl K91 (ab10112) at 2 µg
Lysates/proteins at 1 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size : 12 kDa
Observed band size : 11 kDa (why is the actual band size different from the predicted?)
Predicted Molecular weight11KDa
ChIP - Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4627 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
References for Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
This product has been referenced in:
- Gatta R & Mantovani R Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters. Cell Cycle 9: (2010). ChIP; Human.Read more (PubMed: 20505338) »
- Tjeertes JVet al. Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells. EMBO J 28:1878-89 (2009). WB; Human.Read more (PubMed: 19407812) »
See all 7 publications for this product
Publishing research using ab4627? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"