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Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.
Histone H4 Acetylation Assay Kit (ab115103) allows the user to measure global histone H4 acetylation at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|10X Lysis Buffer||1 x 5ml||1 x 10ml|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Acetylated Histone H4 Control (60 µg/mL)||1 x 10µl||1 x 20µl|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Capture Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Detection Antibody, 400 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 5ml||1 x 10ml|
|Extraction Buffer||1 x 8ml||1 x 16ml|
|Histone Buffer||1 x 0.5ml||1 x 1ml|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115103 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"