Overview

  • Product nameAnti-Histone H4 antibody
    See all Histone H4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H4
  • Tested applicationsSuitable for: IP, ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 10-59. Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805 ) around the acetylation site of lysine 12 (L-G-KA-G-G).
    Database link: P62805

  • Positive control
    • Human breast carcinoma tissue and Jurkat cell extracts treated with TSA (400nM, 24hours).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab61255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

Anti-Histone H4 antibody images

  • Histone H4 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab61255.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 13kDa; Histone H4

  • ab61255 at 1/50 - 1/100 dilution staining Histone H4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Histone H4 antibody (ab61255) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with TSA (400nM, 24hours)
    Lane 2 : Jurkat cell extracts treated with TSA (400nM, 24hours) with the immunising peptide


    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ICC/IF image of ab61255 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61255, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Histone H4 antibody (ab61255)

This product has been referenced in:
  • Sadeghi L  et al. The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast. EMBO Rep 16:1673-87 (2015). WB ; Schizosaccharomyces pombe . Read more (PubMed: 26518661) »
  • Colpitts TM  et al. Dengue virus capsid protein binds core histones and inhibits nucleosome formation in human liver cells. PLoS One 6:e24365 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21909430) »

See all 2 Publications for this product

Product Wall

Yes, the antibody can also recongize the acetylated histone H4. This antibody has been tested this antibody by ELISA and can react with Acetyl peptide and Non-acetyl peptide. The results are here below: Dilution 1:1000 - 1:4...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"