Overview

  • Product name
  • Description
    Rabbit polyclonal to Histone H4
  • Specificity
    Reactivity with other animals is expected owing to the conservation of sequence among Histone H4 from all animals.
  • Tested applications
    Suitable for: IHC-P, ChIP, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Drosophila melanogaster, Plants, Zebrafish, Chlamydomonas reinhardtii, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (N terminal). Synthetic peptide, corresponding to a region between residues 1 and 50 of Human Histone H4 (NP_778224.1)
    Database link: P62805

  • Positive control
    • Histone extract from HeLa cells. IHC-P: Human normal colon FFPE tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 11 kDa).
IP Use at 1-4 µg/mg of lysate.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Histone H4 with ab70701 at 1/200 (1µg/ml). Detection: DAB.
  • IHC image of ab70701 staining Histone H4 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70701, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • All lanes : Anti-Histone H4 antibody (ab70701) at 1/2000 dilution

    Lane 1 : Histone extract from HeLa cells at 50 µg/ml
    Lane 2 : Histone extract from HeLa cells at 15 µg/ml
    Lane 3 : Histone extract from HeLa cells at 5 µg/ml


    Predicted band size : 11 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Almeida M  et al. Epigenetic regulation of EFEMP1 in prostate cancer: biological relevance and clinical potential. J Cell Mol Med 18:2287-97 (2014). ChIP ; Human . Read more (PubMed: 25211630) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (K562)
Specification
K562
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Other
Username

Abcam user community

Verified customer

Submitted Nov 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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