Anti-Histone H4 (asymmetric di methyl R3) antibody (ab194683)

Overview

  • Product name
    Anti-Histone H4 (asymmetric di methyl R3) antibody
    See all Histone H4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H4 (asymmetric di methyl R3)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Dot blot, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (asymmetric di methyl R3). A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
    Database link: P62805

  • Positive control
    • HeLa cell extract. 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 11 kDa.
ICC/IF 1/50 - 1/200.
Dot blot Use at an assay dependent concentration.
IP 1/50 - 1/100.

Target

Images

  • Immunocytochemical analysis of 293T cells labeling Histone H4 (asymmetric di methyl R3) with ab194683 at 1/50 dilution (red) and DAPI (blue)

  • Dot-blot analysis of methylation peptides using ab194683

  • All lanes : Anti-Histone H4 (asymmetric di methyl R3) antibody (ab194683) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Histone H4 protein

    Predicted band size: 11 kDa

References

ab194683 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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