Anti-Histone H4 (citrulline R3) antibody (ab81797)


  • Product nameAnti-Histone H4 (citrulline R3) antibody
  • Description
    Rabbit polyclonal to Histone H4 (citrulline R3)
  • SpecificityThis antibody reacts specifically with human Histone H4 Citrulline at position 3 (10kDa). The antibody does not cross react with free H4 nor with other citrullinated proteins.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (N terminal) (citrulline R3). Synthetic peptide derived from N terminal region of Human Histone H4, containing Citrulline at position 3.
    Database link: P62805
    (Peptide available as ab171570)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab81797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 10 kDa.Can be blocked with Histone H4 peptide (ab171570).
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/10000.


  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Involvement in diseaseChromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.
  • Sequence similaritiesBelongs to the histone H4 family.
  • Post-translational
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
    Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST2H4 antibody
    • Hist4h4 antibody
    • Histone H4 antibody
    see all

Anti-Histone H4 (citrulline R3) antibody images

  • ELISA control of the specificity of ab81797 using H4-3cit peptide, H4-Arg3 peptide and H3-cit2, 8, 17 peptide.

References for Anti-Histone H4 (citrulline R3) antibody (ab81797)

ab81797 has not yet been referenced specifically in any publications.

Product Wall

The only western blot data we are aware of for this antibody is the data at the following link, which we received from a customer. It is not encouraging.

Read More

Application Western blot
Sample Human Cell lysate - whole cell (primary cells)
Loading amount 20 µg
Specification primary cells
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Submitted Mar 11 2011