Anti-Histone H4 (di methyl K20) antibody (ab118513)

Overview

  • Product nameAnti-Histone H4 (di methyl K20) antibody
    See all Histone H4 primary antibodies
  • Description
    Mouse monoclonal to Histone H4 (di methyl K20)
  • SpecificityMinimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at the recommended concentrations.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, a wide range of other speciesDoes not react with: Saccharomyces cerevisiae, Tetrahymena
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 17-28 (di methyl K20) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    RHRK(Me2)VLRDNIQGC

  • Positive control
    • IHC-P: Human skin tissue. WB: HeLa acid extracts. IP: HeLa cell lysates. ICC/IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab118513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.01 - 0.1 µg/ml. Predicted molecular weight: 11 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-Histone H4 (di methyl K20) antibody images

  • ab118513 staining Histone H4 (di methyl K20) in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab118513 at 1µg/ml and ab6046 (anti beta Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green) and Alexa Fluor® 594 Goat anti-Rabbit secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • ab118513 at 5 µg/ml staining Histone H4 (di methyl K20) in Formalin-Fixed, Paraffin-Embedded Human Skin tissue.

References for Anti-Histone H4 (di methyl K20) antibody (ab118513)

ab118513 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Caenorhabditis elegans Cell (Gut and Embryo)
Specification Gut and Embryo
Fixative Paraformaldehyde
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Submitted Sep 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"