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Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (di-methyl K20) has been described as repressive chromatin domain and involved in DNA damage response.
Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095) enables the user to measure global di-methylation of histone H4K20. The global H4K20 di-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global di-methyl histone H4K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115095 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Color Developer||1 x 5ml||1 x 10ml|
|Detecting Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Signal Enhancer||1 x 120µl||1 x 240µl|
|Signal Report Solution||1 x 5µl||1 x 10µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115095 has not yet been referenced specifically in any publications.