Recombinant
RabMAb

Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)

Overview

  • Product name
    Anti-Histone H4 (methyl R67) antibody [EPR18914]
    See all Histone H4 primary antibodies
  • Description
    Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67)
  • Host species
    Rabbit
  • Specificity
    This antibody detects mono methyl, asymmetric di methyl and symmetric di methyl arginine 67.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    within Human Histone H4 aa 50 to the C-terminus (methyl R67). The exact sequence is proprietary.
    Database link: P62805

  • Positive control
    • WB: Human testis lysate; RAW 264.7, F9, NIH/3T3 and HeLa whole cell lysates; mouse spleen lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR18914
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab177827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

Images

  • Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lane 1 & 2: 4 seconds; Lane 3: 15 seconds.

     

References

ab177827 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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