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Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of the proper higher order structure of DNA and is consequently essential for chromosome condensation. It is also involved in gene silencing.
Histone H4 (mono-methyl K20) Quantification Kit (Colorimetric) (ab115093) enables the user to measure global mono-methylation of histone H4K20. The global H4K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. ab115093 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Color Developer||1 x 5ml||1 x 10ml|
|Detecting Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Signal Enhancer||1 x 120µl||1 x 240µl|
|Signal Report Solution||1 x 5µl||1 x 10µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab115093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115093 has not yet been referenced specifically in any publications.