Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)

Overview

  • Product nameHistone H4 (pan-methyl K20) Quantification Kit (Fluorometric)See all Histone H4 (K20 methylation) kits ...
  • Tests
    1 x 96 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    1 ng/well
  • Range
    10 ng/well - 2000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, as well as functioning in gene silencing. H4 (di-methyl K20) has been described as another repressive chromatin domain and is involved in the DNA damage response. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.

    Abcam's Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100) enables the user to measure global mono-, di- and tri-methylation of histone H4K20. The global H4K20 pan-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global pan-methyl histone H4K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115100 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115100 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)

    ab115100 has not yet been referenced specifically in any publications.

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