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Abcam's Histone H4 Total Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states. It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Abcam's histone modification quantification kit series.
The kit is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|1000X Capture Antibody||1 x 5µl||1 x 10µl|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|2000X Signal Reporter||1 x 6µl||1 x 12µl|
|8-Well Assay Strips (with Frame)||1 x 6 units||1 x 12 units|
|Color Developer||1 x 5ml||1 x 10ml|
|Enhancer Solution||1 x 6µl||1 x 12µl|
|Histone Assay Buffer||1 x 4ml||1 x 8ml|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab156909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Total H4 per mg of extracted histones from HeLa control cells (C) or cells treated with 10 micromolar sodium butyrate (But) (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).
Total H4 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Histone nuclear extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the ODs generated from Histone H4 are measured.
ab156909 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"