Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)

Overview

  • Product nameHistone H4 (tri-methyl K20) Quantification Kit (Colorimetric)See all Histone H4 (K20 methylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    5 ng/well
  • Range
    20 ng/well - 2000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.

    Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097) enables the user to measure global tri-methylation of histone H4K20. The global H4K20 tri-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global tri-methyl histone H4K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115097 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115097 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)

    ab115097 has not yet been referenced specifically in any publications.

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